University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center
5 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
James E. East *1, Wenji Sun *1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland
Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.
Advanced Biology
ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center
ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
Advanced Biology
Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy
Michael S. Lee 1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center
Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy
Advanced Biology
Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
Dominique R. Bollino 1, Eric A. Legenzov 2, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center, 2Center for Biomedical Engineering and Technology, University of Maryland School of Medicine
Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
Advanced Biology
Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
Susannah C. Shissler 1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland
Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
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