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Centre Léon Bérard

3 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

Johann Gout^1,2,3,4,5, Roxane M. Pommier^1,2,3,4,5, David F. Vincent^1,2,3,4,5, Bastien Kaniewski^1,2,3,4,5, Sylvie Martel^1,2,3,4,5, Ulrich Valcourt^*1,2,3,4,5, Laurent Bartholin^*1,2,3,4,5

¹INSERM U1052, Centre de Recherche en Cancérologie de Lyon, ²CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Université Lyon 1, ⁵Centre Léon Bérard

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

Cancer Research

Evaluation of the Feasibility, Safety, and Accuracy of an Intraoperative High-intensity Focused Ultrasound Device for Treating Liver Metastases

Aurélien Dupre^1,2, David Melodelima², David Perol³, Yao Chen¹, Jérémy Vincenot², Jean-Yves Chapelon², Michel Rivoire^1,2

¹Department of Surgical Oncology, Centre Léon Bérard, ²Applications des ultrasons à la thérapie (LabTAU), Institut national de la santé et de la recherche médicale (INSERM), Centre Léon Bérard, Université Lyon 1, Université Lyon, ³Biostatistics and Treatment Evaluation Unit, Centre Léon Bérard

Here, we present an ablate-and-resect prospective study to evaluate the feasibility, safety, and accuracy of intraoperative high-intensity focused ultrasound ablation in patients undergoing hepatectomy for liver metastases.

Biology

A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

Kawtar Arizkane^1,2,3, Kevin Geistlich^1,2,3,4, Laurine Moindrot^1,2,3, Emma Risson^1,2,3, Sandrine Jeanpierre^1,2,3,4, Léa Barral^1,2,3, Anaëlle Bobard^1,2,3, Giulio Menegazzi⁵, Thibault Voeltzel^1,2,3, Véronique Maguer-Satta^1,2,3, Sylvain Lefort^1,2,3

¹Centre de Recherche en Cancérologie de Lyon, ²Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Centre Léon Bérard, ⁵Department of Experimental and Clinical Biomedical Sciences Mario Serio, Università degli Studi di Firenze

Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events.