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Zhongshan Hospital of Dalian University

2 ARTICLES PUBLISHED IN JoVE

Biology

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Yanning Wu¹, Shuo Wang¹, Chunying Li^1,2,3

¹Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, ²Cardiovascular Research Institute, Wayne State University School of Medicine, ³Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

Cystic fibrosis transmembrane conductance regulator (CFTR), an epithelial chloride channel, has been reported to interact with various proteins and regulate important cellular processes; among them the CFTR PDZ motif-mediated interactions have been well documented. This protocol describes methods we developed to assemble a PDZ-dependent CFTR macromolecular signaling complex in vitro.

Biology

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Miquella G. Chavez^1,2, Jimmy Hu¹, Kerstin Seidel¹, Chunying Li^1,3, Andrew Jheon¹, Adrien Naveau^4,5,6, Orapin Horst^1,7, Ophir D. Klein^1,8

¹Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, ²Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, ³Department of Pathology and Research Center, Zhongshan Hospital of Dalian University, ⁴Université Paris Descartes, Sorbonne Paris Cite, UMR S872, ⁵Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S872, ⁶INSERM U872, ⁷Division of Endodontics, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, ⁸Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco

The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.