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Stanford School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Joseph Dragavon¹, Carolyn Sinow², Alexandra D. Holland¹, Abdessalem Rekiki¹, Ioanna Theodorou³, Chelsea Samson⁴, Samantha Blazquez¹, Kelly L. Rogers⁵, Régis Tournebize^1,6,7, Spencer L. Shorte¹

¹Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, ²Department of Radiation Oncology, Stanford School of Medicine, ³Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, ⁴Vanderbilt School of Medicine, ⁵The Walter & Eliza Hall Institute of Medical Research, ⁶Unité INSERM U786, Institut Pasteur, ⁷Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur

Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.

Developmental Biology

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Alyssa Klein^1,2,3, Bethel Bayrau^1,2,3, Yifei Miao^1,2,3,4,5, Mingxia Gu^1,2,3,4,5

¹Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, ²Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, ³Stanford Cardiovascular Institute, Stanford School of Medicine, ⁴Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, ⁵Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads.