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Heart Research Center Goettingen

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

Eva Wagner^*1,2,3, Sören Brandenburg^*1,2, Tobias Kohl^1,2, Stephan E. Lehnart^1,2,3,4

¹Heart Research Center Goettingen, ²Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, ³German Center for Cardiovascular Research (DZHK) partner site Goettingen, ⁴BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine

In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.

Biochemistry

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Andreas Andric¹, Eva Wagner¹, Anne Heberle¹, Max Holzknecht¹, Alexander K. H. Weiss¹

¹Research Institute for Biomedical Aging Research, University of Innsbruck

This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.