Entrar

University of Toronto, University Health Network

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

Venkatesh Krishnan¹, Robert Clark¹, Marina Chekmareva², Amy Johnson¹, Sophia George³, Patricia Shaw⁴, Victoria Seewaldt^4,5, Carrie Rinker-Schaeffer¹

¹Section of Urology, Department of Surgery, The University of Chicago, ²Department of Pathology, Robert Wood Johnson Medical School, ³Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre, University Health Network, ⁴Department of Laboratory Medicine and Pathobiology, Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre, University of Toronto, University Health Network, ⁵Departments of Medicine, Pharmacology, and Cancer Biology, Duke University Medical Center

We outline a protocol that implements both in vivo and ex vivo approaches to study ovarian cancer colonization of peritoneal adipose tissues, particularly the omentum. Furthermore, we present a protocol to quantitate and analyze immune cell-structures in the omentum known as milky spots, which promote metastases of peritoneal adipose.

Medicine

Acquisition and Semi-Automated Analysis of Respiratory Muscle Surface Electromyography

Antenor Rodrigues^1,2, Luc Janssens^3,4, Daniel Langer^4,5, Umi Matsumura⁶, Dmitry Rozenberg^7,8, Laurent Brochard^1,2, W. Darlene Reid^2,9,10

¹Keenan Centre for Biomedical Research, Li Ka Shing Knowledge Institute, Unity Health Toronto, ²Interdepartmental Division of Critical Care Medicine, University of Toronto, ³Faculty of Engineering Technology, Electrical Engineering, KU Leuven, ⁴Department of Rehabilitation Sciences, Faculty of Movement and Rehabilitation Sciences, Research Group for Rehabilitation in Internal Disorders, KU Leuven, ⁵Respiratory Rehabilitation and Respiratory Division, University Hospital Leuven, ⁶Department of Health Sciences, Nagasaki University Graduate School of Biomedical Sciences, ⁷Department of Medicine, Temerty Faculty of Medicine, Division of Respirology, University of Toronto, University Health Network, ⁸Toronto General Hospital Research Institute, University Health Network, ⁹Department of Physical Therapy, University of Toronto, ¹⁰KITE, Toronto Rehabilitation Institute, University Health Network

Here, we describe a protocol to record and analyze respiratory electromyography (EMG) signals. It includes the anatomic references for placing the EMG electrodes over several respiratory muscles, removing electrocardiographic noise from the EMG signals, and acquiring the EMG root mean square (RMS) and onset timing of activity.