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Universität Heidelberg

4 ARTICLES PUBLISHED IN JoVE

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Biology

Contractility Measurements on Isolated Papillary Muscles for the Investigation of Cardiac Inotropy in Mice
Sebastian Uhl 1, Marc Freichel 1, Ilka Mathar 1
1Pharmakologisches Institut, Universität Heidelberg

Murine left ventricular papillary muscle can be used to investigate cardiac contractility in vitro. This article describes in detail the isolation and experimental protocols to study cardiac contractile characteristics.

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Developmental Biology

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Irene U. Wacker 1,2, Lisa Veith 3, Waldemar Spomer 2,4, Andreas Hofmann 2,4, Marlene Thaler 5, Stefan Hillmer 6, Ulrich Gengenbach 2,4, Rasmus R. Schröder 1,2,3
1Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility, Universität Heidelberg

This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.

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Immunology and Infection

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Volodymyr Tsvilovskyy 1, Alejandra Solis-Lopez 1, Kathrin Öhlenschläger 1, Marc Freichel 1
1Institute of Pharmacology, Ruprecht-Karls Heidelberg University

Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca2+ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase.

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JoVE Core

Cardiac Response to β-Adrenergic Stimulation Determined by Pressure-Volume Loop Analysis
Rebekka Medert *1,2, Lucas Bacmeister *1,2,3, Sebastian Segin *1,2,4, Marc Freichel 1,2, Juan E. Camacho Londoño 1,2
1Pharmakologisches Institut, Ruprecht-Karls-Universität Heidelberg, 2DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, 3Department of Internal Medicine III, University of Heidelberg, 4Department of Anesthesiology, University Hospital RWTH Aachen

Here we describe a cardiac pressure-volume loop analysis under increasing doses of intravenously infused isoproterenol to determine the intrinsic cardiac function and the β-adrenergic reserve in mice. We use a modified open-chest approach for the pressure-volume loop measurements, in which we include ventilation with positive end-expiratory pressure.

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