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Shriners Hospitals for Children-Boston

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

William J. McCarty^1,2, Ljupcho Prodanov^1,2, Shyam Sundhar Bale^1,2, Abhinav Bhushan^1,2, Rohit Jindal^1,2, Martin L. Yarmush^1,2, O. Berk Usta^1,2

¹Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, ²Shriners Hospitals for Children-Boston

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

Bioengineering

Mouse Model of Pressure Ulcers After Spinal Cord Injury

Suneel Kumar¹, Yuying Tan¹, Martin L. Yarmush^1,2, Biraja C. Dash³, Henry C. Hsia³, Francois Berthiaume¹

¹Department of Biomedical Engineering, Rutgers University, ²Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children, ³Department of Surgery, Yale School of Medicine, Yale University

Here, we describe a simple method to induce clinically relevant skin pressure ulcers (PUs) in a mouse model of spinal cord injury (SCI). This model can be used in pre-clinical studies to screen for different therapeutics for healing PUs in SCI patients.