로그인

Shriners Hospitals for Children-Boston

2 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
William J. McCarty 1,2, Ljupcho Prodanov 1,2, Shyam Sundhar Bale 1,2, Abhinav Bhushan 1,2, Rohit Jindal 1,2, Martin L. Yarmush 1,2, O. Berk Usta 1,2
1Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, 2Shriners Hospitals for Children-Boston

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

image

Bioengineering

Mouse Model of Pressure Ulcers After Spinal Cord Injury
Suneel Kumar 1, Yuying Tan 1, Martin L. Yarmush 1,2, Biraja C. Dash 3, Henry C. Hsia 3, Francois Berthiaume 1
1Department of Biomedical Engineering, Rutgers University, 2Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children, 3Department of Surgery, Yale School of Medicine, Yale University

Here, we describe a simple method to induce clinically relevant skin pressure ulcers (PUs) in a mouse model of spinal cord injury (SCI). This model can be used in pre-clinical studies to screen for different therapeutics for healing PUs in SCI patients.

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2024 MyJoVE Corporation. 판권 소유