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INM-Leibniz Institute for New Materials

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

¹INM-Leibniz Institute for New Materials, ²Department of Physics, University of Saarland

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

Engineering

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

Justus Hermannsdörfer¹, Niels de Jonge^1,2

¹INM-Leibniz Institute for New Materials, ²Department of Physics, University of Saarland

This protocol describes the operation of a liquid flow specimen holder for scanning transmission electron microscopy of AuNPs in water, as used for the observation of nanoscale dynamic processes.

Biology

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Patricia Blach^1,2, Sercan Keskin¹, Niels de Jonge^1,2

¹INM-Leibniz Institute for New Materials, ²Department of Physics, Saarland University

Presented here is a protocol for labeling membrane proteins in mammalian cells and coating the sample with graphene for liquid-phase scanning transmission electron microscopy. The stability of the samples against the damage caused by radiation can also studied with this protocol.