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Lausanne University Hospital

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

Ruslan Cusnir¹, Philipp Steinmann², Marcus Christl³, François Bochud¹, Pascal Froidevaux¹

¹Institute of Radiation Physics, Lausanne University Hospital, ²Federal Office of Public Health, Bern, Switzerland, ³Laboratory of Ion Beam Physics, ETH Zurich

The technique of diffusive gradients in thin films (DGT) is proposed for speciation studies of plutonium. This protocol describes diffusion experiments probing the behavior of Pu(IV) and Pu(V) in presence of organic matter. DGTs deployed in a karstic spring allow assessment of the bioavailability of Pu.

Behavior

Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice

Jessica Lavier^1,2, Manon Beaumann³, Steeve Ménetrey³, Lucia Mazzolai¹, Anne-Christine Peyter³, Maxime Pellegrin¹, Grégoire P. Millet²

¹Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), ²Institute of Sport Sciences, Faculty of Biology and Medicine, University of Lausanne, ³Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital

High-intensity training in hypoxia is a protocol that has been proven to induce vascular adaptations potentially beneficial in some patients and to improve athletes' repeated sprint ability. Here, we test the feasibility of training mice using that protocol and identify those vascular adaptations using ex vivo vascular function assessment.

Genetics

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

Antonio Mancarella¹, Francesco A. Procopio¹, Tilmann Achsel², Elisa De Crignis³, Brian T. Foley⁴, Giampietro Corradin⁵, Claudia Bagni², Giuseppe Pantaleo¹, Cecilia Graziosi¹

¹Division of Immunology and Allergy, Lausanne University Hospital, ²Department of Fundamental Neuroscience, University of Lausanne, ³Department of Biochemistry, Erasmus Medical Center, ⁴Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, ⁵Department of Biochemistry, University of Lausanne

RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.

Medicine

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Martino Guiotto^*1,4, Silvia Palombella^*2, Stefania Brambilla², Lee Ann Applegate³, Mathis Riehle⁴, Andrew Hart⁴, Wassim Raffoul¹, Pietro Giovanni di Summa¹

¹Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, ²Cell and Tissue Engineering Lab, IRCCS Ospedale Galeazzi Sant'Ambrogio, ³Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, Lausanne University Hospital, ⁴Centre for the Cellular Microenvironment, University of Glasgow

Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.