John Innes Centre, Norwich Research Park
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
Susan Schlimpert 1, Klas Flärdh 2, Mark J. Buttner 1
1Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, 2Department of Biology, Lund University
Streptomyces are characterized by a complex life cycle that has been experimentally challenging to study by cell biological means. Here we present a protocol to perform fluorescence time-lapse microscopy of the complete life cycle by growing Streptomyces venezuelae in a microfluidic device.
Biology
Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
Thomas R. Vincent 1, James Canham 1, Masatsugu Toyota 2,3,4, Marieta Avramova 1, Sam T. Mugford 5, Simon Gilroy 2, Anthony J. Miller 1, Saskia Hogenhout 5, Dale Sanders 1
1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park
This protocol outlines a simple method for analyzing calcium signals in plants generated by feeding hemipteran insects, such as aphids. Arabidopsis thaliana transformed with the GFP calcium biosensor GCaMP3 allow for the real-time in vivo imaging of calcium dynamics with a high temporal and spatial resolution.
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