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Central Arkansas Veterans Healthcare System

2 ARTICLES PUBLISHED IN JoVE

Genetics

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Isabelle R. Miousse¹, Igor Koturbash¹, Marie-Cécile Chalbot², Martin Hauer-Jensen³, Ilias Kavouras², Rupak Pathak³

¹Department of Occupational and Environmental Health, University of Arkansas for Medical Sciences, ²Department of Environmental Health Sciences, University of Alabama at Birmingham, ³Division of Radiation Health, University of Arkansas for Medical Sciences

This protocol describes techniques for the quantification and characterization of chromosomal aberrations in vitro in RAW264.7 mouse macrophages after treatment with ambient air particulate matter.

Genetics

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Rupak Pathak¹, Igor Koturbash², Martin Hauer-Jensen^1,3

¹Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, ²Department of Environmental Health, Fay W. Boozman School of Public Health, University of Arkansas for Medical Sciences, ³Surgical Service, Central Arkansas Veterans Healthcare System

The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.