Central Arkansas Veterans Healthcare System
2 ARTICLES PUBLISHED IN JoVE
Genetics
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Isabelle R. Miousse 1, Igor Koturbash 1, Marie-Cécile Chalbot 2, Martin Hauer-Jensen 3, Ilias Kavouras 2, Rupak Pathak 3
1Department of Occupational and Environmental Health, University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences, University of Alabama at Birmingham, 3Division of Radiation Health, University of Arkansas for Medical Sciences
This protocol describes techniques for the quantification and characterization of chromosomal aberrations in vitro in RAW264.7 mouse macrophages after treatment with ambient air particulate matter.
Genetics
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
Rupak Pathak 1, Igor Koturbash 2, Martin Hauer-Jensen 1,3
1Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, 2Department of Environmental Health, Fay W. Boozman School of Public Health, University of Arkansas for Medical Sciences, 3Surgical Service, Central Arkansas Veterans Healthcare System
The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.
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