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Japan Advanced Institute of Science and Technology

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface

Kosuke Okeyoshi¹, Kensuke Osada², Maiko K. Okajima¹, Tatsuo Kaneko¹

¹Japan Advanced Institute of Science and Technology, ²Department of Bioengineering, School of Engineering, The University of Tokyo

A method for the drying-induced self-integration of megamolecular biopolymers on the air-liquid crystalline interface is provided here. This methodology will be useful not only for understanding the macroscopic potentials of biopolymers, but also as an evaluation method for soft materials in biomedical and environmental fields.

Bioengineering

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Ruchika ¹, Toshifumi Tshukahara¹, Manish Biyani^1,2

¹Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, ²BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.