Determination of the Glycogen Content in CyanobacteriaAlice De Porcellinis 1, Niels-Ulrik Frigaard 2, Yumiko Sakuragi 3
1Carlsberg Research Laboratory, 2Department of Biology, University of Copenhagen, 3Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen
Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.
