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ICPMR

2 ARTICLES PUBLISHED IN JoVE

Biology

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Matthew V. N. O'Sullivan¹, Fei Zhou¹, Vitali Sintchenko¹, Fanrong Kong¹, Gwendolyn L. Gilbert¹

¹Centre for Infectious Diseases and Microbiology, University of Sydney

An inexpensive, high throughput method for simultaneous detection of up to 43 molecular targets is described. Applications of mPCR/RLB include microbial typing and detection of multiple pathogens from clinical samples.

Medicine

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Chayanika Biswas^*1, Sharon C-A. Chen^*1,2,3, Catriona Halliday^1,2, Elena Martinez¹, Rebecca J. Rockett¹, Qinning Wang¹, Verlaine J. Timms¹, Rajat Dhakal¹, Rosemarie Sadsad¹, Karina J. Kennedy⁴, Geoffrey Playford^4,5, Deborah J. Marriott⁶, Monica A. Slavin⁷, Tania C. Sorrell^1,3, Vitali Sintchenko^1,2,3

¹Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, ²Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, ³Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, ⁴Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, ⁵Infection Management Services, Australian National University Medical School, ⁶Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, ⁷Department of Infectious Diseases, Peter MacCallum Cancer Centre

This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.