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United States Department of Agriculture - Agricultural Research Service

1 ARTICLES PUBLISHED IN JoVE

Genetics

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Ling Huang^*1, Xiaohui Deng^*1, Ronghua Li¹, Yanshi Xia¹, Guihua Bai², Kadambot H.M. Siddique³, Peiguo Guo¹

¹College of Life Sciences, Guangzhou University, ²Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, ³The UWA Institute of Agriculture, University of Western Australia

Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.