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United States Department of Agriculture - Agricultural Research Service

1 ARTICLES PUBLISHED IN JoVE

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Genetics

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Ling Huang *1, Xiaohui Deng *1, Ronghua Li 1, Yanshi Xia 1, Guihua Bai 2, Kadambot H.M. Siddique 3, Peiguo Guo 1
1College of Life Sciences, Guangzhou University, 2Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, 3The UWA Institute of Agriculture, University of Western Australia

Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.

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