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Erasmus MC University Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Simone Punt 1, Robert J. Baatenburg de Jong 2, Ekaterina S. Jordanova 1,3
1Department of Pathology, Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam, VU Medical Center

Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.

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Medicine

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model
Panithi Sukho 1,2,3, Geesien S.A. Boersema 4, Nicole Kops 5, Johan F. Lange 4, Jolle Kirpensteijn 1,6, Jan Willem Hesselink 1, Yvonne M. Bastiaansen-Jenniskens 5, Femke Verseijden 1,5
1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 2Department of Otorhinolaryngology, Erasmus MC University Medical Center, 3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, 4Department of Surgery, Erasmus MC University Medical Center, 5Department of Orthopaedics, Erasmus MC University Medical Center, 6Hill's Pet Nutrition Inc.

The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

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Medicine

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way
Yassine Aaboubout 1,2, Elisa M. Barroso 1,3,4, Mahesh Algoe 1, Patricia C. Ewing-Graham 1, Ivo ten Hove 3,6, Hetty Mast 3, José A. Hardillo 2, Aniel Sewnaik 2, Dominiek A. Monserez 2, Stijn Keereweer 2, Brend P. Jonker 3, Cornelia G. F. van Lanschot 2, Roeland W. H. Smits 2, Maria R. Nunes Soares 1,4, Lars Ottevanger 1, Sanne E. Matlung 1, Paul A. Seegers 5, Vera van Dis 1, Robert M. Verdijk 1, Eppo B. Wolvius 3, Peter J. Caspers 4, Tom C. Bakker Schut 4, Robert J. Baatenburg de Jong 2, Gerwin J. Puppels 4, Senada Koljenović 1
1Department of Pathology, Erasmus MC University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC University Medical Center, 3Department of Oral and Maxillofacial Surgery, Erasmus MC University Medical Center, 4Department of Dermatology, Erasmus MC University Medical Center, 5PALGA foundation, The nationwide network and registry of histo- and cytopathology, 6Department of Oral and Maxillofacial Surgery, Leiden University Medical Center

The goal of this protocol is to provide a clear overview of specimen-driven intraoperative assessment of resection margins. It is encouraged to implement this protocol to improve patient care at other institutes.

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