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City University of Hong Kong, Hong Kong

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat

Francis A. M. Manno^*1, Sinai H. C. Manno^*2,3,4, Victor Ma⁵, Fernando A. Barrios¹, William C Cho⁵, Shuk Han Cheng^2,3,6, Condon Lau⁴

¹Instituto de Neurobiología, Universidad Nacional Autónoma de México, México, ²State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Hong Kong, ³Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, ⁴Department of Physics, City University of Hong Kong, Hong Kong, ⁵Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, ⁶Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong

Here, we present a protocol to establish a replicable conductive hearing loss induction via surgical tympanic membrane puncture and verification by otoscope visualization and behavioral assessment by clap startle.

JoVE Core

Assessing the Autonomic and Behavioral Effects of Passive Motion in Rats using Elevator Vertical Motion and Ferris-Wheel Rotation

Francis A. M. Manno^1,2, Leilei Pan³, Yuqi Mao³, Yang Su³, Sinai H. C. Manno^2,4,5, Shuk Han Cheng^4,5,6, Condon Lau², Yiling Cai³

¹School of Biomedical Engineering, Faculty of Engineering, University of Sydney, ²Department of Physics, City University of Hong Kong, ³Department of Nautical Injury Prevention, Faculty of Navy Medicine, Second Military Medical University, ⁴State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, ⁵Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, ⁶Department of Materials Science and Engineering, City University of Hong Kong

Protocols are presented to assess the autonomic and behavioral effects of passive motion in rodents using elevator vertical motion and Ferris-wheel rotation.