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The Chaim Sheba Medical Center

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Marianne Strazza¹, Inbar Azoulay-Alfaguter¹, Michael Peled¹, Adam Mor^1,2

¹Department of Medicine, New York University School of Medicine, ²Department of Pathology, New York University School of Medicine

This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.

Immunology and Infection

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Michael Peled¹, Marianne Strazza², Adam Mor²

¹Pulmonary Department, The Chaim Sheba Medical Center, ²Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center

Here, we present a protocol for co-immunoprecipitation and an on-bead enzymatic activity assay to simultaneously study the contribution of specific protein domains of plasma membrane receptors to both enzyme recruitment and enzyme activity.