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Baker Heart & Diabetes Institute

3 ARTICLES PUBLISHED IN JoVE

JoVE Core

Preparation of Aligned Steel Fiber Reinforced Cementitious Composite and Its Flexural Behavior

Ru Mu¹, Luansu Wei¹, Xiaowei Wang¹, Hui Li¹, Longbang Qing¹, Jian Zhou¹, Quanming Zhao²

¹School of Civil and Transportation Engineering, Hebei University of Technology, ²School of Information Engineering, Hebei University of Technology

This protocol describes an approach for manufacturing aligned steel fiber reinforced cementitious composite by applying a uniform electromagnetic field. Aligned steel fiber reinforced cementitious composite exhibits superior mechanical properties to ordinary fiber reinforced concrete.

Bioengineering

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Tatjana Michel¹, Antonia Link¹, Meike-Kristin Abraham^1,2, Christian Schlensak¹, Karlheinz Peter^2,3, Hans-Peter Wendel¹, Xiaowei Wang^2,3, Stefanie Krajewski¹

¹Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, ²Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, ³Department of Medicine, Monash University

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

Neuroscience

In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy

Amanda M Sweeney^*1, Virginia Plá^*1, Ting Du¹, Guojun Liu¹, Qian Sun¹, Sisi Peng¹, Benjamin A. Plog¹, Benjamin T. Kress¹, Xiaowei Wang¹, Humberto Mestre¹, Maiken Nedergaard^1,2

¹Center for Translational Neuromedicine, University of Rochester Medical Center, ²Center for Translational Neuromedicine, University of Copenhagen

Transcranial optical imaging allows wide-field imaging of cerebrospinal fluid transport in the cortex of live mice through an intact skull.