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Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Ombretta Guardiola¹, Gennaro Andolfi¹, Mario Tirone^2,3, Francescopaolo Iavarone¹, Silvia Brunelli², Gabriella Minchiotti¹

¹Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, ²School of Medicine and Surgery, University of Milano-Bicocca, ³Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute

This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.

JoVE Journal

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Giorgia Careccia^*1,2, Federica Colombo^*1,3, Mario Tirone¹, Alessandra Agresti¹, Marco E. Bianchi^1,2, Samuel Zambrano^*1,2, Emilie Vénéreau^*1

¹Chromatin Dynamics Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University, ³Department of Electronics, Information and Bioengineering, Politecnico di Milano

Skeletal muscle differentiation is a highly dynamic process, which particularly relies on nuclear positioning. Here, we describe a method to track nuclei movements by live cell imaging during myoblast differentiation and myotube formation and to perform a quantitative characterization of nuclei dynamics by extracting information from automatic tracking.