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2 ARTICLES PUBLISHED IN JoVE

Medicine

Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration

Abhishek Kumar¹, Arjun Mohan¹, Samjot S. Dhillon², Kassem Harris²

¹Division of Pulmonary and Critical Care Medicine, State University of New York, Buffalo, ²Interventional Pulmonology Section, Division of Medicine, Roswell Park Cancer Institute, State University of New York, Buffalo

Substernal thyroid lesions are common, and need to be differentiated from malignancy. Obtaining percutaneous fine needle biopsy is not possible due to its retrosternal location. This article proposes a protocol for biopsy of substernal thyroid lesions using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration (EBUS-TBNA).

Developmental Biology

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Leighton H. Duncan^1,5, Mark W. Moyle^1,5, Lin Shao^1,5, Titas Sengupta^1,5, Richard Ikegami^1,5, Abhishek Kumar^4,5, Min Guo^4,5, Ryan Christensen^4,5, Anthony Santella^2,5, Zhirong Bao^2,5, Hari Shroff^4,5, William Mohler^3,5, Daniel A. Colón-Ramos^1,5,6

¹Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, ²Developmental Biology Program, Sloan Kettering Institute, ³Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, ⁴Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, ⁵WormGUIDES.org, ⁶Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.