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University Hospital Essen Hufelandstr

2 ARTICLES PUBLISHED IN JoVE

Genetics

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Johannes Korth^1,2, Helene Sertznig², Siegfried Moyrer², Adrian Atilla Nicolas Doevelaar^2,3, Timm Henning Westhoff³, Nina Babel³, Oliver Witzke⁴, Andreas Kribben¹, Ulf Dittmer², Marek Widera²

¹Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, ²Institute for Virology, University of Duisburg-Essen, University Hospital Essen, ³Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, ⁴Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

In this manuscript, a protocol is presented to perform FACS-based measurement of BK-polyomavirus transcriptional activity by using HEK293T cells transfected with a bidirectional reporter plasmid expressing tdTomato and eGFP. This method further allows to quantitatively determine the influence of novel compounds on viral transcription.

Medicine

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

Decheng Yin^*1,2, Jian Fu^*1,3, Ida Allabauer¹, Oliver Witzke⁴, Song Rong⁵, André Hoerning¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, ⁵Department of Nephrology, Hannover Medical School

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.