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Dana-Farber/Boston Children's Cancer and Blood Disorders Center

1 ARTICLES PUBLISHED IN JoVE

Medicine

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Francisco J. Molina Estevez^*1, Tyler D. Mathews^*1, Alessandra Biffi^1,2, Marco Peviani^1,2

¹Dana-Farber/Boston Children's Cancer and Blood Disorders Center, ²Harvard Medical School

We present a flow cytometry method to identify simultaneously different cell types retrieved from mouse brain or spinal cord. This method could be exploited to isolate or characterize pure cell populations in neurodegenerative diseases or to quantify the extent of cell targeting upon in vivo administration of viral vectors or nanoparticles.