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Fralin Biomedical Research Institute at Virginia Tech Carilion

2 ARTICLES PUBLISHED IN JoVE

Behavior

Inducing Post-Traumatic Epilepsy in a Mouse Model of Repetitive Diffuse Traumatic Brain Injury

¹Fralin Biomedical Research Institute at Virginia Tech Carilion, ²School of Neuroscience, Virginia Tech, ³Department of Biological Sciences, Virginia Tech

This systematic protocol describes a new animal model of post-traumatic epilepsy after repetitive mild traumatic brain injury. The first part details steps for traumatic brain injury induction using a modified weight drop model. The second part provides instructions on the surgical approach for single- and multi-channel electroencephalographic data acquisition systems.

A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow

Samantha Howerton^1,2, Yanping Liang¹, Jennifer Hammel^1,3, Benjamin Purow⁴, Jennifer Munson^1,3

¹Fralin Biomedical Research Institute at Virginia Tech Carilion, ²Translational Biology, Medicine, and Health Graduate Program, Virginia Tech, ³Department of Biomedical Engineering & Mechanics, Virginia Tech, ⁴Department of Neurology, University of Virginia School of Medicine

We present a method for replicating the glioma tumor microenvironment at the invasive front that incorporates interstitial fluid flow. This model is a hyaluronan-collagen I hydrogel in a tissue culture insert where a fluid pressure head can be applied. Invasion can be quantified, and cells can be isolated or lysed.