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Yuhang District Maternity and Child Health Care Hospital

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Fengwei Zhang¹, Yanan Wang², Xuechai Shang¹, Shanshan Wang¹, Rong Xiao¹, Hongjuan Zhou¹, Long Cai¹

¹Central Laboratory, Hang Zhou Red Cross Hospital, ²Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.