Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 VirusesFengwei Zhang 1, Yanan Wang 2, Xuechai Shang 1, Shanshan Wang 1, Rong Xiao 1, Hongjuan Zhou 1, Long Cai 1
1Central Laboratory, Hang Zhou Red Cross Hospital, 2Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital
This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.
