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Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

2 ARTICLES PUBLISHED IN JoVE

Behavior

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication

Allain-Thibeault Ferhat¹, Nicolas Torquet², Anne-Marie Le Sourd¹, Fabrice de Chaumont³, Jean-Christophe Olivo-Marin³, Philippe Faure², Thomas Bourgeron¹, Elodie Ey¹

¹Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, ²Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, ³Bio Image Analysis, CNRS URA 2582, Institut Pasteur

Mouse ultrasonic vocalizations are used as proxies to model the genetic bases of vocal communication deficits in mouse models for neuropsychiatric disorders. The present protocol describes three experimental contexts that reliably elicit ultrasonic vocalizations from pups (throughout development) and adult mice (same-sex interactions, male-estrus female interactions).

Bioengineering

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Géraud Heckler¹, Christelle Aigueperse¹, Liza Hettal¹, Quentin Thuillier¹, Fabrice de Chaumont², Stéphane Dallongeville², Isabelle Behm-Ansmant¹

¹Université de Lorraine, CNRS, IMoPA F54000 Nancy France, ²Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.