Download Icy and the "Substructure Analyzer" Protocol on Icy Website
1:30
Opening the Protocol
2:03
Interacting with the Workflow on Icy
2:49
Merging of the Channels of an Image
4:57
Segmentation of the Regions of Interest
7:42
Fluorescent Signal Detection and Analysis
9:25
Run the Protocol
10:35
Representative Results
13:48
Conclusion
Transcrição
Substructure analyzer is a user friendly workflow that performs automatic analysis of multiple process microscopy metrics. It doesn't mean zero tons of open source software Icy and also using machine functionalities. Importantly, this workflow is
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We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.