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University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

A Protocol to Set Up Needle-Free Connector with Positive Displacement on Central Venous Catheter in Intensive Care Unit

Thomas Clavier^1,2, Sébastien Franchina¹, Antoine Lefevre-Scelles¹, Philippe Gouin¹, Emmanuel Besnier^1,2, Benoit Veber¹

¹Department of Anesthesiology and Critical Care, Rouen University Hospital, ²Normandie Univ, UNIROUEN, INSERM U1096

We present a protocol to show the installation of a needle-free connector with positive displacement on a central venous catheter.

Neuroscience

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Martin Pedard¹, Mohamad El Amki¹, Antoine Lefevre-Scelles^1,2, Vincent Compère^1,2, Hélène Castel¹

¹University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France, ²Department of Anesthesiology and Critical Care, Rouen University Hospital, Rouen, France

We described in this protocol a standardized subarachnoid hemorrhage (SAH) mouse model by a double injection of autologous whole-blood into the cisterna magna. The high degree of standardization of the double-injection procedure represents a middle-to-acute model of SAH with relative safety regarding mortality.