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Jagiellonian University Medical College

3 ARTICLES PUBLISHED IN JoVE

Biology

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Gabriela Gorczyca¹, Kamil Wartalski², Zbigniew Tabarowski³, Malgorzata Duda¹

¹Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow, ²Department of Histology, Jagiellonian University Medical College, ³Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow

Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.

Immunology and Infection

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Magdalena Zemelka-Wiacek¹, Monika Majewska-Szczepanik², Pawel Gajdanowicz¹, Marian Szczepanik³

¹Department of Clinical Immunology, Wroclaw Medical University, ²Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, ³Chair of Biomedical Sciences, Faculty of Health Sciences, Jagiellonian University Medical College

Contact hypersensitivity (CHS) is a murine experimental model of allergic contact dermatitis (ACD). CHS is based on sensitization with reactive hapten by painting the shaved skin of the chest and abdomen, with a subsequent ear skin challenge with a diluted hapten, causing a swelling reaction that is assessed in various ways.

Immunology and Infection

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Aneta Babula^*1,2, Izabela Siemińska^*1,3, Monika Baj-Krzyworzeka¹

¹Department of Clinical Immunology, Jagiellonian University Medical College, ²Doctoral School of Medical and Health Sciences, Jagiellonian University, ³Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.