Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in RiceDawei Xu *1,2, Palash Chandra Mondol *1, Muhammad Uzair 1, Matthew R. Tucker 3, Dabing Zhang 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.
