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Ludwig-Maximilians University Munich (LMU)

3 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca²⁺ Transients and L-Type Calcium Current

Philipp Tomsits^*1,2,3, Dominik Schüttler^*1,2,3, Stefan Kääb^1,2, Sebastian Clauss^*1,2,3, Niels Voigt^*4,5,6

¹Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), ²Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), ³Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), ⁴Institute of Pharmacology and Toxicology, University Medical Center Göttingen, ⁵Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), ⁶Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen

Mouse models allow studying key mechanisms of arrhythmogenesis. For this purpose, high quality cardiomyocytes are necessary to perform patch-clamp measurements. Here, a method to isolate murine atrial and ventricular myocytes via retrograde enzyme-based Langendorff perfusion, which allows simultaneous measurements of calcium-transients and L-type calcium current, is described.

Medicine

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice

Philipp Tomsits^1,2,3, Kavi Raj Chataut^1,2, Aparna Sharma Chivukula^1,2, Li Mo^1,2, Ruibing Xia^1,2, Dominik Schüttler^1,2,3, Sebastian Clauss^1,2,3

¹Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University Munich (LMU)

Here we present a step-by-step protocol for a semiautomated approach to analyze murine long-term electrocardiography (ECG) data for basic ECG parameters and common arrhythmias. Data are obtained by implantable telemetry transmitters in living and awake mice and analyzed using Ponemah and its analysis modules.

Medicine

Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice

Philipp Tomsits^*1,2,4, Aparna Sharma Chivukula^*1,2, Kavi Raj Chataut^*1,2, Agus Simahendra³, Ludwig T. Weckbach^1,2,3, Stefan Brunner¹, Sebastian Clauss^1,2,4

¹Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, ⁴Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU)

Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice.