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The Children’s Hospital of Philadelphia

2 ARTICLES PUBLISHED IN JoVE

Biology

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Marni J. Falk^1,2, Meera Rao^*1, Julian Ostrovsky^*1, Evgueni Daikhin¹, Ilana Nissim¹, Marc Yudkoff^1,2

¹Department of Pediatrics, The Children's Hospital of Philadelphia, ²Department of Pediatrics, University of Pennsylvania

Stable isotopic profiling by gas chromatography mass spectrometric analysis of intermediary metabolic flux is described in the nematode, Caenorhabditis elegans. Methods are detailed for assessing isotopic enrichment in carbon dioxide, organic acids, and amino acids following isotope exposure either during development on agar plates or during adulthood in liquid culture.

Behavior

Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

Manuela Lavorato^*1, Neal D. Mathew^*1, Nina Shah¹, Eiko Nakamaru-Ogiso^1,2, Marni J. Falk^1,2

¹Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, ²Department of Pediatrics, University of Pennsylvania Perelman School of Medicine

This study presents protocols for two semi-automated locomotor activity analysis approaches in C. elegans complex I disease gas-1(fc21) worms, namely, ZebraLab (a medium-throughput assay) and WormScan (a high-throughput assay) and provide comparative analysis among a wide array of research methods to quantify nematode behavior and integrated neuromuscular function.