Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini BioreactorsArtem Eremeev 1,3, Lilia Belikova 1,2, Evgeny Ruchko 1, Egor Volovikov 1,5, Olga Zubkova 1, Alexy Emelin 4, Roman Deev 4, Olga Lebedeva 1,3, Alexandra Bogomazova 1,3, Maria Lagarkova 1,3
1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University
Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.
