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Max-Planck-Institute for Experimental Medicine

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Athanasios Katsalifis 1, Angela Maria Casaril 1, Constanze Depp 2, Carlos Bas-Orth 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine

This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.

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