Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric IndicatorAthanasios Katsalifis 1, Angela Maria Casaril 1, Constanze Depp 2, Carlos Bas-Orth 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine
This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.
