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Center for Drug Evaluation and Research, Food and Drug Administration

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Todd Atherly³, Marilyn N. Martinez⁴, Donna A. Volpe⁵, Jamie Kopper², Karin Allenspach^2,3, Jonathan P. Mochel^1,3

¹Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, ³3D Health Solutions Inc., ⁴Office of New Animal Drug Evaluation, Center for Veterinary Medicine, Food and Drug Administration, ⁵Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, Food and Drug Administration

Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.