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The State University of New York

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

¹Department of Biological and Vision Sciences, College of Optometry, The State University of New York

Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.

Biochemistry

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Gabrielle R. Budziszewski¹, M. Elizabeth Snell¹, Tiffany R. Wright¹, Miranda L. Lynch¹, Sarah E. J. Bowman^1,2

¹National High-Throughput Crystallization Center, Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, The State University of New York

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Bioengineering

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Cong Xu^*1, Siyu He^*1, Yuefei Zhu¹, Gazelle Crasto², Caroline Chen¹, Morgan L. Clay³, Yeh-Hsing Lao³, Kam W. Leong¹

¹Department of Biomedical Engineering, Columbia University, ²College of Dental Medicine, Division of Periodontics, Columbia University, ³Department of Pharmaceutical Sciences, University at Buffalo, The State University of New York

This protocol presents a method for generating vascular organoids using human pluripotent stem cells.