Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever BorreliaAnne Berthold *1, Marie-Line Faucillion *2, Ingela Nilsson 2, Maryna Golovchenko 3, Vett Lloyd 1, Sven Bergström 2, Natalie Rudenko 3
1Department of Biology, Mt. Allison University, 2Department of Molecular Biology, Umeå University, 3Biology Centre Czech Academy of Sciences, Institute of Parasitology
In vitro culture is a direct detection method for the presence of living bacteria. This protocol describes methods for the culture of diverse Borrelia spirochetes, including those of the Borrelia burgdorferi sensu lato complex, relapsing fever Borrelia species, and Borrelia miyamotoi. These species are fastidious and slow growing but can be cultured.
