Preparation of Complete Barbour-Stoenner-Kelly (BSK)-II Medium
2:48
Culturing Borrelia with or Without Antibiotics and Long-Term Storage
5:48
Isolation of Spirochetes from Environmental or Clinical Sources
7:30
Isolation of Monoclonal Populations of B. burgdorferi Sensu Lato Spirochetes and B. miyamotoi from Liquid Cultures by Cultivation on Solid Media
8:39
Results: Culturing of Diverse Borrelia Spirochetes
9:23
Conclusion
Transcrição
Borrelia is a genus of spirochete bacteria that encompasses three major kinds. The Lyme Borreliosis group, the Relapsing Fever group, and the less well-characterized group found in reptiles. Many Borrelia species are pathogenic.
The culture
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In vitro culture is a direct detection method for the presence of living bacteria. This protocol describes methods for the culture of diverse Borrelia spirochetes, including those of the Borrelia burgdorferi sensu lato complex, relapsing fever Borrelia species, and Borrelia miyamotoi. These species are fastidious and slow growing but can be cultured.