Mobile Device-assisted Dietary Ecological Momentary Assessments for the Evaluation of the Adherence to the Mediterranean Diet in a Continuous Manner

Resumo

Presented here is a study protocol aimed at monitoring continuous adherence to the Mediterranean diet (MedDiet) by means of ecological momentary assessments. The method evaluates the intake of key food groups of the MedDiet and calculates an index of adherence.

Resumo

Mobile device-assisted dietary ecological momentary assessments (EMAs) have emerged as a new tool allowing the evaluation of dietary intake in real time, in a real-world setting and in a continuous manner. They have the potential to minimize recall bias, participant, and investigator burden, and decrease economic and time investment while maximizing ecological validity.

We developed a set of EMAs aimed at evaluating continuous adherence to the MedDiet. Four multiple-choice EMAs are sent daily in a randomized manner from a total of eight questions. The EMAs enquire about the consumption of 11 key food groups of the Mediterranean diet in the last 24-48 h in a semi-quantitative way. EMAs capture the daily frequency of consumption of fruits, vegetables, and extra virgin olive oil on different days of the week. Additionally, EMAs capture the weekly frequency of consumption of whole grain products, sugary drinks, nuts, legumes, sweets, fish and seafood, and red and processed meats. A designed scoring system behind the EMAs extracts the percentage of adherence to the MedDiet recommendations and calculates a quality index of the diet every week. Individualized reports are sent periodically to the volunteers highlighting the strengths and weaknesses of their diet. EMAs are also expected to have a behavioral effect, reinforcing the choice of Mediterranean foods.

Introdução

The Mediterranean diet (MedDiet) is a dietary pattern associated with longevity and multiple health benefits. High adherence to the MedDiet has been related to a decreased risk of overall mortality, cardiovascular disease, overall cancer incidence, neurodegenerative disease and diabetes¹. In particular, the MedDiet, based on seasonal and local products, is characterized by a high intake of plant-based food (fruit, vegetables, legumes, nuts, and unrefined cereals) and a moderate intake of fish, eggs, dairy, and poultry. Olive oil represents the main source of fat. The consumption of saturated fatty acids is low, with very low consumption of red and processed meats, sweets, and processed foods. The MedDiet is also characterized by a moderate intake of ethanol, mostly in the form of wine consumed during meals².

The evaluation of diet is complex and challenging. The correct evaluation of dietary practices and MedDiet adherence in the study of nutrition is the key in attempting to find associations between this dietary pattern and its health outcomes. The traditional methods used to assess diet are food frequency questionnaires, food diaries and 24-h dietary recall. They have been broadly used in nutrition epidemiology and in clinical nutrition, however, they are highly subjected to misreporting, recall bias and depend on the participant's capacity to estimate food content and portion size. These traditional dietary assessment methods are time-consuming, expensive and represent an important burden for both participant and researcher^3,4. To overcome these limitations, there is a need to reformulate traditional dietary assessment. The goal of the dietary assessment is to achieve a balance between the collection of accurate and reliable data with the resources consumed and the burden for the participant³. Several researchers have developed complementary approaches to evaluate adherence to the MedDiet. These approaches calculate composite dietary scores that result from the combination of different dietary characteristics associated with the MedDiet². The first MedDiet score was created in 1995 by Trichoupoulou et al. (1995)⁵ and includes a total of 8 components. The score assessed the frequency of consumption of 7 food groups: vegetables, fruits and nuts, legumes, cereals, meat and meat products, milk and dairy products and alcohol intake. The eighth component was a fat quality measurement; the ratio between monounsaturated and saturated fatty acid (MUFA/SFA)⁵. One of the most used MedDiet assessment questionnaires, the MEDAS (Mediterranean Diet Adherence Screener) was developed during the PREDIMED study (Prevención con Dieta Mediterránea) with the aim of controlling dietary intervention compliance⁶. The MEDAS is a validated 14 item dietary screener which considers additional items compared to the first MedDiet score such as the type of oil used in cooking, consumption of sugar in drinks and sweets and the consumption of the typical Spanish spiced tomato sauce known as "sofrito". The questionnaire was found to be useful in evaluating the adherence to the MedDiet especially in time-limited settings such as large epidemiological studies and general clinical practice⁶.

The widespread availability of new technologies and the changes in how people use them have created the opportunity to incorporate these innovations into dietary assessment. They offer the chance to capture the complexity of food intake while overcoming the aforementioned limitations of traditional methods. In this context, ecological momentary assessments (EMAs) have been developed as a tool to use new technologies in performing repeated sampling of an individual's current behavior and experience⁷. The introduction of EMAs into dietary assessment can improve accuracy, ecological validity, and data robustness while minimizing reporting and recall bias and decreasing participant and research burdens. Additionally, the use of EMAs allows for the continuous assessment of diet; through the observation of fluctuations across time, observation of within-person changes, and the modeling of these variations. EMAs have the potential to minimize the reactivity bias, yield higher rates of compliance and lower the occurrence of missing data^4,7,8. In summary, the major advantages associated with the use of EMAs are: (1) the collection of data as it occurs in the natural environment, (2) real time or near real time data collection rather than retrospective survey, thereby avoiding recall bias, and (3) repeated sampling, which allows for the study of behaviors and experiences over a given time period^4,9. The use of EMAs to assess dietary intake is increasing and several clinical trials have used them to collect dietary information. The type of data collected in these studies include: the frequency of meals and snacks, the consumption of predefined food groups and beverages, and the recording of food images^4,7.

To the best of our knowledge, the EMA approach has never been used to study the adherence to the MedDiet. The aim of the present study was to develop a set of mobile device assisted EMAs to continuously assess the adherence to the MedDiet. To do so, we developed a set of 8 mobile device-assisted EMAs to measure the consumption of 11 food groups, including the assessment of those typical of the MedDiet (olive oil, fruit, vegetables, etc.) along with the intake of food groups that represent a typically low consumption in the MedDiet (processed and red meat, sugary drinks, etc.).

Protocolo

This protocol demonstrates how to continuously assess adherence to the MedDiet by means of tailored EMAs. This protocol has been reviewed and approved by the local ethics committee of the Hospital del Mar: CEIm-PSMAR (reference number: 2019/8972).

1. Study design: Sampling protocol

1. Determine the number of days to assess the dietary intake; a minimum of 1 week is required to obtain the first score and, therefore, ensure that the total number of days is adapted on a 7-days basis (e.g., 4 weeks of assessment to obtain the MedDiet adherence score over a month).
   NOTE: The determination of the number of days is closely related to the aim of the study. In studies monitoring the adherence to the MedDiet exclusively, short periods of time such as 2 weeks is recommended. In the case of intervention studies aimed at improving MedDiet adherence, longer periods of evaluation are recommended.
2. Determine the number of waves for the study (e.g., two monitoring periods of two weeks separated by a month). In the case of longitudinal studies, the evaluation of MedDiet adherence can be integrated in the repeated sampling of volunteers.
   ​NOTE: We leave the decision of the number of waves to the researcher's criteria. This decision is likely going to be subject to a set of appraisal criteria such as the aim of the survey in the context of the study, the type of volunteers among others. Based on the literature, most of the studies monitor during only one period, while some other included up to 8 waves of data collection. Studies with more than one monitoring period had smaller duration than those with only one monitoring period⁸.
3. Determine the schedule of the delivery of EMAs (e.g., every evening at 21:00h).
4. Determine the latency of EMAs; as in the amount of time given to the volunteers to answer the questions (e.g., 2 h).

2. Selection of participants

1. Define the inclusion criteria for the participants' selection.
   1. Ensure that participants possess a smartphone.
      NOTE: Alternatively, the study can provide smartphones in cases where participants do not have one.
   2. Ensure participants have good internet connection on their smartphone.
      NOTE: Alternatively, the study can provide internet connection to the participants.
2. Define the exclusion criteria for the participant's selection.
   1. Do not include participants who are illiterate and/or do not have digital skills.
   2. Do not include participants residing in environments without internet connection.
3. Select study participants based on the inclusion exclusion criteria.

3. Meeting with the participants before the assessment

1. Schedule an introduction session with the participants.
2. Explain in detail how to answer daily questions, emphasizing how they will receive notifications, how to respond to EMAs and the amount of time required to respond.
   1. Inform them that they will receive the questions in their phone via SMS. The SMS has attached a specific link that leads to the questionnaire. Each link is specific for the volunteer and the day.
3. Introduce participants to the 8 EMA questions, explain which foods belong and do not belong to each category and describe the serving sizes when appropriate (Table 1).
4. To ensure the correct comprehension of EMAs, prepare a pilot test with participants, providing them with an example of a diet and enabling them to respond to EMAs based on the presented example.
   1. Prepare an example of a diet of an individual describing specifically the types of food consumed and portion sizes. An example has been included as supplementary material.
   2. Provide the example to the participants via email and ask them to read it carefully.
   3. Give the volunteers the 8 questions included in the EMA survey on an online form and ask them to answer them based on the diet example given. The online form consists of 8 multiple-choice questions and volunteers must be identified with their id study number.
   4. Correct the questions and return a personalized feedback via email to each volunteer, with an explanation of the mistakes done.

Question Nº	Food categories enquired	Food included	Food excluded of the group	Servings (s)
Q1	Extra virgin olive oil	Extra virgin olive oil	Olive oil	Not applicable
	(EVOO)
Q2	Vegetables	Salads, cooked vegetables, “sofrito”, frozen vegetables...	Potato, sweet potatoes, peas...	1 s = 200g grams
				½ s = Side dish
Q3	Fruit	All fruit including raw, cooked…	Juices, yogurt with fruits, jam…	1 s = 1 medium piece, 1 slice of melon/ watermelon, 2-3 small pieces…
Q4	Whole-grain food	All whole grain products	Refined cereals and non-whole grain multigrain products	Not applicable
Q5	Sugary drinks (including juices)	Soft drinks with and without sugar, natural and packaged juice	Water and alcoholic drinks	1 s = 1 glass
Q6	Legumes	Dried and cooked legumes, peas, tofu…	Corn	1 s = 150 g
Q6	Nuts	All nuts	Dried fruit	1 s = 25 g
Q7	Sweets	Home-made and industrial baking		Not applicable
Q8	Fish and seafood	All types: raw, frozen, canned, smoked…	Surimi and derivates	1 s fish = 100-150 g
				1 s seafood = 200 g
Q8	Red meat	Bovine, game, viscera, duck…	Chicken, turkey, lean pork cuts	1 s = 100 – 150 g
Q8	Processed meat	Sausages, ham, mince, or cured meat…	-	1 s = 50 g
Q: Question; s: Serving

Table 1: List of food categories enquired, detailing examples of food products included and excluded, and the size of the serving reported.

4. Measurement of the adherence to the MedDiet using EMAs.

1. Send daily four randomized EMAs via a link attached in a SMS (Figure 1) following the weekly frequency outlined in Table 2 for the period of time established in the study. In this experiment the platform LimeSurvey (https://www.limesurvey.org) was used for the EMA delivery and for data storage.

Nº	Food group	Type of question		Interval of enquiry	Weekly frequency of the question
Question
Q1	EVOO	Qualitative		Last 24 hours	4 times
		(Yes/No and usage)
Q2	Vegetables	Semi-Quantitative (Nº of servings)		Last 24 hours	4 times
Q3	Fruit	Semi-Quantitative (Nº of servings)		Last 24 hours	4 times
Q4	Whole-grain food	Qualitative		Last 24 hours	3 times
		(Yes/No)
Q5	Sugary drinks (including juice)	Semi-Quantitative (Nº of servings)		Last 24 hours	3 times
Q6	Legumes	Semi-Quantitative (Nº of servings)		Last 48 hours	3 times
Q6	Nuts	Semi-Quantitative (Nº of servings)		Last 48 hours	3 times
Q7	Sweets	Qualitative		Last 48 hours	3 times
		(Yes/No)
Q8	Fish and seafood	Semi-Quantitative (Nº of servings)		Last 48 hours	4 times
Q8	Red meat	Semi-Quantitative (Nº of servings)		Last 48 hours	4 times
Q8	Processed mead	Semi-Quantitative (Nº of servings)		Last 48 hours	4 times

Table 2: Description of the EMAs, type of question, interval of enquiry and frequency.

Figure 1: Example of four daily EMAs received by the participants. Please click here to view a larger version of this figure.

2. During the first days of the study check that participants are replying to EMAs:
   1. Log in to the questionnaire administrator interface.
   2. Click on the answer space and search with the number of volunteer whether they have answered and whether the answer is complete (indicated with a green tick) or incomplete (indicated with a red cross).
   3. Contact participants who are not replying to detect technical problems, if any.

5. Assess the weekly adherence to the MedDiet

1. After 7 days of receiving EMAs, calculate a score of adherence to the MedDiet.
   1. Download all completed answer from the survey website.
      1. Log in into the questionnaire administrator interface.
      2. Click on the menu to answers and then click to export answers.
      3. Select the data format to export and click on "only completed answers".
      4. Click to export to download the file.
   2. Open the excel file and sort columns by order of day and number of volunteers.
   3. Check for duplicated answers and eliminate, if any.
   4. Using a spreadsheet, sort all the answers given by each volunteer and translate the answer obtained into frequencies of consumption of the enquired food groups as detailed in Table 3.
2. Compare the frequencies of consumption obtained for each individual with the ones described in the MedDiet awarding the score for each food category as detailed in Table 3.
3. Add up the scores of all enquired food groups to obtain the final score. The score has a maximum of 11 points. Additionally, translate the score into a percentage and classify into the following MedDiet adherence levels:
   Weekly score ≤ 5 points (≤ 45%): Low adherence to the MedDiet
   Weekly score > 5 and < 8 points (< 73%): Moderate adherence to the MedDiet
   Weekly score ≥ 8 points (≥ 73%): High adherence to the MedDiet
4. Assess the compliance rate of the participants by means of the calculation of the accuracy of data of the week using the following formula:
   
   Number of EMAs answered: total number of EMA prompts completely answered
   Number of EMAs delivered: total number of EMA prompts send to the participants: 4 prompts x 7 days of the week =28
   NOTE: Accuracies ≥80% are considered acceptable. Low accuracy percentages indicate low compliance of the participants and hence the quality of the data obtained is poor and probably not representative.
5. Send personalized feedback to the participant by email; including the weekly score and the score achieved in each food category. Extend the assessment for a total of 4 consecutive weeks to obtain the monthly score by adding the scores achieved in the 4 weeks period (maximum 44 points). The monthly calculated score can be classified in the following MedDiet adherence levels:
   Monthly score ≤20 points (≤45%): Low adherence to the MedDiet
   Monthly score >20 and <32 points (<73%): Moderate adherence to the MedDiet
   Monthly score ≥32 points (≥73%): High adherence to the MedDiet
6. Send personalized feedback to the participant including the monthly score.

Nº Question	Food enquired	Punctuation	Fulfillment with the recommendations	Recommendations *
Q1	EVOO	1	≥ 75 % 1	Use of EVOO as the main source of fat in each meal
		0.5	≥ 50%
		0	< 50%
Q2	Vegetables	1	≥ 75 % 1	2-3 servings/day
		0.5	≥ 50
		0	< 50%
Q3	Fruit	1	75 % 1	3-4 servings/day
		0.5	≥ 50%
		0	< 50%
Q4	Whole-grain food	1	≥ 66 % 2	Preference for whole-grain foods
		0	< 66%
Q5	Sugary drinks	1	≥ 66 % 3	Occasional and moderate consumption
		0	< 66%
Q6	Legumes	1	≥ 2 times/week	2-4 servings/week
		0	< 2 times/week
Q6	Nuts	1	≥ 3 times/week	3-7 servings/week
		0.5	≥ 2 times/week
		0	< 2 times/week
Q7	Sweets	1	≥ 66 % 3	Occasional and moderate consumption
		0	< 66%
Q8	Fish and seafood	1	≥ 2 times/week	2-3 servings/week
		0	< 2 times/week
Q8	Red meat	1	≤ 1 time/ week 4	Occasional and moderate consumption
		0	> 2 times/week
Q8	Processed meat	1	≤ 1 time/ week 4	Occasional and moderate consumption
		0	> 1 time/ week
* Recommendations are based on Dietary guidelines for the Spanish population (Spanish Society on Community Nutrition, December 2016)
1. We considered a good adherence to the recommendation when subjects complied with more than 75% with the recommendations.
2. We considered a good adherence to the recommendations when whole-grain products where consumed in two or more occasions of the 3 times asked per week.
3. We considered that the consumption was occasional when the intake of sugary drinks and sweets reported was less than once out of the 3 times asked per week.
4. We considered that the consumption was occasional and moderate when the total number of servings reported in the week was ≤1.

Table 3: Items and punctuation criteria to calculate the weekly MedDiet adherence score.

Resultados

The present protocol was used in a proof-of-concept study which included a total of 63 subjects with an age range of 22 to 76 years. The aim of the proof-of-concept study was to compare the adherence to the Mediterranean diet obtained with the proposed EMAs approach with the validated MEDAS test. The present study did not intend to validate the EMAs but to compare both instruments as tools to measure the adherence to the MedDiet, to test its feasibility and the adherence of study participants to a two-week EMAs evaluatio...

Discussão

Here we describe a protocol to assess an individual's adherence to the MedDiet via a mobile-based application. This method uses daily EMAs to capture the dietary pattern and by means of an algorithm, calculates a weekly score representing the degree of adherence to the MedDiet. A positive score is given to the high intake of healthy food items which are characteristic of the MedDiet. Conversely, a negative score is given to the intake of unhealthy food groups, in accordance with the recommendations given for the Span...

Materiais

Name	Company	Catalog Number	Comments
Data processing software (excel)	MS Office	-	Others suitable options like R studio
Google forms	Google	-	Free online software that allows the creation of  surveys and questionnaires to be delivered. It's part of Google's web-based apps sui
Limesurvey platofrm (https://www.limesurvey.org/)	Limesurvey	-	A free software application for conducting online surveys