Name: micro-dissection of rat brain for rna or protein extraction from specific brain region
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

<p class="jove_step">Step 1</p>
<p class="jove_content">Sacrifice the rat according to established protocols. Remove the brain from the skull and rinse it in ice cold DEPC treated Milli Q water to remove any surface blood.</p>
<p class="jove_step">Step 2</p>
<p class="jove_content">Place on cold metal plate. Cut the brain bi-half into right and left hemisphere.<br /><br /><img src="/files/ftp_upload/old/269_2008_7_19_3/269_Figure-01.png" alt="269_Figure-01" width="474" height="224" /></p>
<p style="PADDING-LEFT: 30px"><strong>Figure 1.&nbsp; Th...

<ol>
	<li>Palkovits, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Maps and guide to micro-dissection of the rat brain</em>. , (c.1988).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Brain maps: structure of the rat brain. 3rd revised edition</em>. , (2004).</li></ol>

<p class="jove_content">Different brain regions associate with different neurodegenerative diseases. For example, frontal cortex and striatum relate with Parkinson s disease while hippocampus relates with Alzheimer's disease. Precise micro-dissection of the brain allows us to detect the mRNA and protein changes in diseased region more accurately.</p>

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<td>Microscope</td>
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<td>Olympus</td>
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<td>Metal plate, forceps, blade, etc. Individually wrapped with aluminum foil and heated at 180oC for eight hours to eliminate RNase. Made ice cold before use. </td>
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micro-dissection of rat brain for rna or protein extraction from specific brain region

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

<p class="jove_content">Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.</p>

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

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