June 27th, 2011
•We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.
Tags
Vídeos Relacionados
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados