July 10th, 2013
•We describe a protocol for isolation and purification of neutrophils from mouse bone marrow by density gradient centrifugation and for neutrophil labeling using CellTracker dyes. This represents a simple, fast, reproducible and economical method for obtaining large numbers of neutrophils for downstream functional studies or adoptive transfer and tracking experiments.
Tags
Vídeos Relacionados
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Bone Marrow-derived Macrophage Production
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Tracking Mouse Bone Marrow Monocytes In Vivo
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados