Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
3:17
Reprogramming Cells by Nulceofection Day 9
4:51
Plating Feeder Cells Day 10-11
5:47
Plating Cells on Feeder Cells and Changing Medium Day 12
6:41
Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
7:46
Conclusion
Here we describe a protocol for generating human induced pluripotent stem cells from peripheral blood using an episome based reprogramming strategy and histone deacetylase inhibitors.