January 12th, 2015
•We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.
Tags
Vídeos Relacionados
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
Dissection and Culture of Mouse Embryonic Kidney
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados