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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

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12:37 min

April 14th, 2016

April 14th, 2016

37,576 Views

0:05

Title

0:57

Extraction of Total Nucleic Acid from Cervicovaginal Swabs (Phenol-Chloroform Extraction)

5:52

PCR Amplification of the 16S rRNA Gene V4 Hypervariable Region

9:40

Library Pooling and High-Throughput Sequencing

10:30

Evaluation of Nucleic Acid Extraction and 16S rRNA Gene PCR Amplification

11:35

Conclusion

Transcrição

We describe an efficient, robust, and cost effective method for extracting nucleic acid from swabs for characterization of bacterial communities using 16S rRNA gene amplicon sequencing. The method allows for a common processing approach for multiple sample types and accommodates a number of downstream analytic processes.

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